Fahrenheit was born in the city of Danzig (Gdańsk), Pomeranian Voivodeship in the Polish–Lithuanian Commonwealth, but lived most of his life in the Dutch Republic. The Fahrenheits were a German Hanse merchant family who had lived in several Hanseatic cities. Fahrenheit's great-grandfather had lived in Rostock, and research suggests that the Fahrenheit family originated in Hildesheim.

Daniel Gabriel began training as a merchant in Amsterdam after his parents died on 14 August 1701 from eating poisonous mushrooms. However, Fahrenheit's interest in natural science led him to begin studies and experimentation in that field. From 1717, he traveled to Berlin, Halle, Leipzig, Dresden, Copenhagen, and also to his hometown, where his brother still lived. During that time, Fahrenheit met or was in contact with Ole Rømer, Christian Wolff, and Gottfried Leibniz. In 1717, Fahrenheit settled in The Hague as a glassblower, making barometers, altimeters, and thermometers. From 1718 onwards, he lectured in chemistry in Amsterdam. He visited England in 1724 and was the same year elected a Fellow of the Royal Society. Fahrenheit died in The Hague and was buried there at the Kloosterkerk (Cloister Church).

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