Of the institutions listed, the university with most Nobel Laureates by affiliation is the University of Cambridge with 120. The University of California, Berkeley has 107, the University of Chicago has 100 and the University of Oxford has 72. Harvard University, U.S., has the highest number of Laureates by affiliation with 160.

The University of Cambridge is a collegiate public research university in Cambridge, United Kingdom. Founded in 1209 and granted a royal charter by King Henry III in 1231, Cambridge is the 2nd-oldest university in the English-speaking world and the world's 4th-oldest surviving university.

This list of Nobel Laureates by university affiliation is counted consistently by application of some clear rules. The list counts Nobel laureates as equal individuals including those with prize shares but not if they received the same subject prize more than once.

The academic affiliations fall into three groups, 1) alumni (graduates and attendees), 2) long-term academic staff, and 3) short-term academic staff. Graduates are defined as those who hold Bachelor's, Master's and Doctorate degrees but not those with honorary degrees or posthumous degrees. Attendees are enrolled students who did not complete their courses of study. "Long-term academic staff" consists of tenured and equivalent academic positions, while "short-term academic staff" consists of lecturers (without tenure), postdoctoral researchers, visiting professors or visiting scholars.

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