In Eastern Christianity, an iconostasis (Greek: "εἰκονοστάσιον") is the solid screen of stone, wood, or metal, usually separating the sanctuary from the nave. Normally, the iconostasis has three doors in it. The two single doors to the right and left are called "deacons' doors" or "angel doors" and they usually have on them icons of sainted deacons. The central double doors are the "holy doors".

The iconostasis always includes the icon of the Incarnation (mother with child) on the left side of the holy doors and the second coming of Christ the “Pantocrator” (“Christ in majesty”) on the right. The sacrament of the Eucharist revealed through the doors between the two main icons, is thus the manifestation of Christ in the church during the time between his two comings.

Icons of the four Evangelists, the Annunciation, and the Last Supper are set over the royal doors themselves. Representations of the archangels Gabriel and Michael, and either sainted deacons like Saint Stephen or Saint Lawrence, the Twelve Apostles, the feasts of the church, and the prophets of the Old Testament are arranged on the iconostasis in complicated patterns, with all figures facing the holy doors.

The veneration of icons came under attack during the Iconoclastic Controversy of 725–843, but the Eastern church finally recognized icons as the main form of representing divine revelation and as a pictorial history of the Christian mystery to be contemplated by the faithful.

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