The Basilica of St. James (Slovak: Bazilika svätého Jakuba) is a Gothic church in Levoča, Prešovský kraj, Slovakia. Building began in the 14th century. It is a Catholic parish church, dedicated to James the Apostle.

The interior features several Gothic altars, including as the main altar the world's tallest wooden altar at 18.62 metres (61.1 ft) by the workshop of Master Paul of Levoča, completed in 1517. The church, the second largest in Slovakia, also houses well-preserved furniture and art work. The steeple dates from the 19th century. The church was added to the United Nations Educational, Scientific and Cultural Organization (UNESCO) World Heritage Site Levoča, Spiš Castle and the associated cultural monuments in 2009. It is also a National Monument. In 2015, Pope Francis declared the church a Basilica minor.

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