With the exception of insects, hummingbirds while in flight have the highest metabolism of all animals – a necessity to support the rapid beating of their wings during hovering and fast forward flight. Their heart rate can reach as high as 1,260 beats per minute, a rate once measured in a blue-throated hummingbird, with a breathing rate of 250 breaths per minute, even at rest. During flight, oxygen consumption per gram of muscle tissue in a hummingbird is about 10 times higher than that measured in elite human athletes

Studies of hummingbirds' metabolisms are relevant to the question of how a migrating ruby-throated hummingbird can cross 800 km (500 mi) of the Gulf of Mexico on a nonstop flight. This hummingbird, like other birds preparing to migrate, stores fat as a fuel reserve, thereby augmenting its weight by as much as 100%, hence increasing potential flying time over open water.

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