In the film The Shawshank Redemption, prisoner Andy Dufresne (Tim Robbins) defies Warden Sam Norton (Bob Gunton) by playing the duettino over the prison's loudspeakers. Ellis Boyd "Red" Redding (Morgan Freeman) remarks in his voice-over narration: "I have no idea to this day what those two Italian ladies were singing about. [...] I'd like to think they were singing about something so beautiful it can't be expressed in words, and it makes your heart ache because of it." This is ironic as the opera characters are singing about a duplicitous love letter to expose infidelity, and Dufresne's wife's affair is the event which indirectly leads to his imprisonment.

The duettino's appearance in the Shawshank Redemption soundtrack earned it a nomination as one of 400 songs in consideration for American Film Institute's list of 100 top movie songs, although it did not win a place on the list.

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